Biochem/physiol Actions

Matrix metalloproteinase-2 (MMP-2) cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln. It is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. It degrades type IV, V, VII, and X collagens. The protein degrades extracellular matrix proteins and acts on several non-matrix proteins such as big endothelial 1 (bET) and β-type calcitonin gene-related peptide (CGRP) promoting vasoconstriction. MMP-2 also cleaves KISS (metastasis suppressor) at a Gly-|-Leu bond. The protein appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of glycogen synthase kinase-3 β (GSK3β). It has been shown to cleave GSK3β in vitro. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to fibroblast growth factor-2 (FGF2) and vitronectin.

General description

Matrix metalloproteinase-2 (MMP-2) which is also known as 72kDa type IV collagenase, 72kDa gelatinase, gelatinase A and CLG4A, belongs to the peptidase M10A family. It contains three fibronectin type-II domains and four hemopexin-like domains. MMP-2 is produced by normal skin fibroblasts. The gene encoding this protein is localized on human chromosome 16q12.2.

Physical form

Lyophilized from 0.22 µm filtered solution in 50 mM Tris, 150 mM NaCl, pH 8.0. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.